Alterations of immune functions and changes of certain redox enzymes and transaminase in liver tissue and echinococcus cysts of mouse infected with echinococcus after treatment with matrine and albendazole
Abstract:In this study the alterations of immune functions and the changes of certain redox enzymes and transaminase in liver tissue and echinococcus cysts of mice infected with echioncoccus granulosus after treatment with matrine(Mat) and albendazole(Alb) were investigated. The echinococcus mouse models established were treated with Mat, Alb alone or in combination with this two drugs for 90 days, and the levels of IL-4, IL-6 and IL-10 in serum were detected through radioimmunoassay (RIA) before and after treatment; as well as the CD~+_4 and CD~+_8 T cells from PMBC classified by flow cytometry (FCM), content and activity of HA and GOT in serum measured by RIA and velocity respectively, the NO and LD content and NOS, LDH activity in hepatic tissue homogenate from echinococcus infected mice and the LD content, LDH and AKP activity in Echinococcus granulosus cyst wall measured by chromatometry. The experimental results demonstrated that the Th2 type cytokines IL-4 ,IL-6 and IL-10 were decreased after treatment, especially the decrease of IL-4 and IL-10 in the case of the combined treatment with Mat and Alb. And the alterations in T lymphocyte subsets were obvious with an increase of CD~+_8 T cells and a concomitant decrease of CD~+_8 T cells, resulting an elevation of the CD~+_4 /CD~+_8 ratio. However, the differences between normal and the treated mice still existed. In the echinococcus cysts walls, the content of LD and the activitiy of LDH and AKP were all increased, especially more obvious in the case of the combined treatment with both drugs, in which the magnitude of decreased. LDH in Mat-treated group was more prominent than that in the Alb-treated group, and LD just decreased only in the Mat-treated group and the combined treatment group. While in the hepatic tissue homogenates, the NO content and the NOS activity decreased in all groups of the treated mice. The serum HA content was decreased apparently in the experimental groups in comparison with the control group, but significant differences of Mat-treated group and combined treatment group with Alb-treated group still existed. The serum GOT content were decreased in Mat-treated and the combined treatment group, but no change was found in the Alb-treated group. It is evident that there are definite alterations in the immune function and changes in redox enzymes and transaminase in the liver tissue and echinococcus cysts of mice infected with echinococcus treated with matrine and albendazole, and that matrine improved remarkably liver function, which also recover hepatocyte scathe better than albendazole does.
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