Abstract：To understand the distribution of the arboviruses in Yunnan province to provide the basis for the measures of control and prevention, the mosquitoes and sera pf patients with fever were collected in Simao and Xishuangbanna prefectures of Yunnan province, and these specimens were stored in liquid nitrogen before testing. For the isolation of viruses, C6/36 cells and the sucking mice were inoculated with specimens obtained, and the isolated viruses were identified by serological assays and molecular techniques, in which the sera of patients with fever and the healthy individuals were tested for the presence of antibodies against the isolated viruses by ELISA and HI assay. It was found that one strain of Sindbis virus from sera of patients with fever in Xishuangbanna prefecture; one strain of Batai virus from Anopheles philippinenisis in Lancang county, and 10 strains of Coltivuses from Culex tritaeniorhynchus , Anopheles sinensis. Anopheles philippinensis, and Anopheles vagus in Chuanyun district of Simao city and Lancang county.The positive rate of antibodies against Sindbis virus was 2.50% (3/120), and that against Batai virus was 4.17% (5/120) in sera of patients with fever in Xishuangbanna prefecture. The total positive rate of antibodies against Sindbis virus was 1.93% (11/571) in the sera of healthy individuals in Xishuangbanna prefecture; and those in Lancang county, Simao city, Jinghong city, Mengla and Menghai county were 4.55% (6/132), 0.89% (1/112), 1,25% (2/160), 1.96% (2/102), and 0% (0/65) respectively. It concludes that the infections due to Sindbis, Batai and Coltiviruses transmitted by mosquitoes were prevalent in Yunnan province with natural infection in healthy individuals. The further investigation for the control and prevention of these infection is necessary.
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