Abstract：To explore the pathological effect of H. pylori on human hepatic cells, this study was conducted to investigate the change of the protein expression of HepG2 caused by H. pylori treatment, and to identify differential expression proteins. H. pylori was co-cultured with HepG2 for 6h, two-dimensional gel eletrophoresis (2-DE) was used to gain the protein pattern of HepG2 cells before and after treated with H. pylori. The differential expression proteins were identified by MALDI-TOF-MS. Seven proteins which were up-regulated in H. pylori treated HepG2 were identified. These proteins included integrin beta-1, protein kinase C alpha, LIM/homeobox protein Lhx1, eIF-2-beta, MAP kinase kinase 3, PINCH protein and Ras-related protein Rab-37, which involved in transcription regulation, metabolism, signaling pathway and so on. H. pylori may exert the pathological effect on HepG2 cells by up-regulating the expression of proteins. It suggests that the differential expression analysis of proteomes be useful to further study of the relation of H. pylori and human hepatobiliary diseases.
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