Abstract:The molecular characteristics of Vibrio cholerae serogroup 139 was investigated in order to search for a rapid detection method of cholera outbreak and the effective means of conducting epidemiological source -tracking. PCR was adopted to detect 4 virulence genes of V.cholerae and the random amplified polymorphic DNA (RAPD) and SPSS software were utilized to conduct DNA polymorphism analysis of the local isolates so that the rapid detection and molecular subtyping of these isolates became possible. It was found that 4 virulence genes were detected from each of the 5 isolates of V.cholerae serogroup 139, and all the isolates could be classified into 3 groups by RAPD, reflecting the relationship of different serogroups of V.cholerae. In conclusion, it is evident that PCR,together with RAPD can be used for the rapid detection of epidemic situation and for the epidemiological source-tracking.
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