Abstract:To obtain the mimic epitope of specific and sensitive diagnostic antigen of Cysticercosis from phage 12 mer peptide library screened with anti lumbricus immunoglobulin (E Ig )of rabbits. The phage mer 12 peptide library was immunoscreened with specific E Ig purified from sera of rabbits in the first round,and the specific C Ig,purified from sera of patients with Cysticercosis, was used to immunoscreen the phage peptide library from the second to the forth round. After 4 rounds of panning, 24 positive plaques were selected and amplified. The reactivity of each clone was examined by ELISA. The positive clones were confirmed by the examination of sera from different patients with parasitie infections. Two specific clones were sequenced and their amino acid sequences were compared with each other. Results showed that 17 of 24 phage clones could bind to the mixed sera of cysticercosis patients, while 6 clones had a same sensitivity in ELISA as the antigen of cysticercus cellulosae performed in Dot~ELISA, but they showed higher specificity than the antigen of cysticercus cellulosae. The amino acid sequences of two clones showed high homology. It indicates that the mimic epitopes play a better role in serodiagnosis of cysticercosis, and the amino acid sequences of two epitopes are the same.