Abstract:In order to provide experimental data for further studies on the immunobiochemical characteristics of protein antigens from different developmental stages of Periplaneta fuliginosa,four groups of soluble protein antigens from Periplaneta fuliginosa,namely,egg antigens,nympha antigens,male and female adult antigens,were analyzed by SDS-PAGE and Enzyme-linked lmmuno-blotting (ELIB).Stripes of the studied antigens were clearly displayed by silver staining after SDS-PAGE.The egg antigens,nympha antigens,male and female adult antigens revealed 13,28,26,41 bands,respectively,of which 2,10,10,13 main bands were found in the respective groups.Most protein components varied in molecular weight between 10 and 97 kDa.The ELIB analysis showed that proteins with immunogenic activities were mainly distributed in a molecular weight range of higher than 43 kDa.The ELIB result also showed an existence of cross-reactive antigenic components among these groups.The number of protein components increases with the advance of development in order of egg-nympha-adult and the protein components tend to get complicated successively.This is considered significant for further study on the biological features of Periplaneta fuliginosa development.