Abstract:To study the effects of the extracellular matrix(ECM) on adherence of the in vitro cultured cells of Schistosoma japomicum and to choose the optimal ECM to promote the adherence of the cultured cells,the 21 days worms of S.japonicum were harvested by perfusion,cell suspension was made by cold digestion and diluted into concentration of 2×10 6 cells/ml.The cell suspension was then inoculated and cultured on the walls of culture flasks that have been smeared previously with bio-matrix from liver and lung of rabbits and collagen from rat tail.The flask without bio-matrix was set up as control.The number of the adherent cells counted timely,from which the rate of cell adherence was calculated.It was found that the rate of cell adherence increased gradually along with the culture times from 8 to 48 hours,and then decreased after 48 hours.At the same culture time,different rates of cell adherence were found in different kinds of ECM.The rates of cell adherence after 48 hours cultivation of collagen of rat tail.bio-matrix from liver and lung from rabbits were 63.27%,48.95% and 45.36% respectively,and the differences among them were significantly.In addition,the difference between the experimental groups and control group was also significant.It concludes that all the 3 ECM shows obvious effects on the adherence of cultured cells of S.paponicum,in which collagen of rat tail shows the most effective effect,and then the bio-matrix from liver and lung of rabbits.