Abstract:Aim To develope an enzyme linked immunosorbent assay for the detection of antibodies to Mycobacterium tuberculosis in the sera of cattle Methods Lipopolysaccharides was isolated from Mycobacterium tuberculosis,bovine and coated an polystyrene microcuvette wells Monoclonal antibody to bovine IgG was conjugated with horseradish peroxidase Results 30 of 46 cattle with positive PPD intradermal test were positive by the ELISA 707 of 750 cattle with negative PPD intradernal test were negative by the ELISA Conclusion This assay is easy and rapid,can be used as an auxiliary test for intradermal test