Abstract:Aim To investigate a simple, rapid and sensitive methods for detecting the antibody against tuberculosis.And while the application value of the methods was identified.Methods We used diazopolystyrene latex as a carrier,and coupled with purificated antigen of Mycobacteriu Tuberculosis,the reagent was made successfully for anti-TB antibody detection by simple slide agglutination test.By this method,the result was obtained in only 3 min.Results The positive rates detected with DLAT,ELISA,or PCR were 48.8%,50.4% or 53.5% in 127 pulmonary tuberculosis patients,respectively,which were not significantly different in same patients (P>0.05).The corresponding rates of the between DLAT and ELISA,or DLAT and PCR were 98.4%,92.1% respectively.Conclusion DLAT is characterized with simple sensitive and rapid method detecting the antibody againast tuberculosis.It is suggested that DLAT can be of choice for serum screening for Mycobacteriu Tuberculosis infection,especially be suitable to apply in field.