Abstract：Aim To isolate HIV-1 from the blood specimen of HIV-infected persons, and study on the features of the iso-lates.Methods PBMCs from 10 heparinized blood specimens of HIV-1 infected persons were collected to isolate the virus with PBMC cocultivation method.Neuramindase was added to the culture medium to raise the HIV isolation rate. The supematant of co-cultures were detected by p24 antigen capture assay and also IFA test was used to identify the isolates. Cell tropism of the HIV-1 isolates was tested by use of H9 and MT4 cell lines, respectively.Results HIV-1 was isolated from 9 of the 10 specimens, the isolation rate could reached 90%.The 9 isolates were used to infect MT4 cells, respectively, and all of them could cause cell syncytias.8 of the isolates showed only a transient infection in MT4 cells ,but the isolate FJ113 could infect MT4 successively,OD values kept no changes even it passed 15 generations, therefore, it is consider as a rapid/high replicating virus.Conclusions Addition fo neuramindase into T cell culture medium may useful to raise HIV isolation rate.Most of HIV-1 isolates in Fujian are M tropic.
1.严延生,等.福建发现首例HIV-2感染者[J].海峡预防医学杂志,1999,5(1):7.
2.严延生,等.福建省艾滋病的流行特征及趋势[J].中华流行病学杂志,1999. 20(1):23.
3.方福德,等.现代医学实验技巧全书(上册)[M].北京医科大学中国协和医科大学联合出版社,1996,F279.
4.曾毅,等.我国首次从艾滋病病人分离到艾滋病病毒(HIV)[J].中华流行病学杂志,1988,3:135.
5.邵一鸣,等.从云南艾滋病毒(HIV)感染者分离HIV[J].中华流行病学杂志,19911,3:129
6.孙中和,等.从中国大陆艾滋病病人血液分离出首株人免疫缺陷病毒[J].中华微生物学和免疫学杂志,1992,1:1.
7.赵永森,等 从一名国内感染的艾滋病病人分离人免疫缺陷病毒(HIV)[J].病毒学报,1994,10(3):216.
8.李关汉,等 HIV-1 SH01株的生物学特征研究[J].中华微生物学和免疫学杂志,1998,18(2):96
9.赵全壁,等 1995年云南瑞丽人免疫缺陷病毒的生物学特性[J].中华实验和临床病毒学杂志,1996,10:4.
10.林旭东,等.我国人免疫缺陷病毒感染者病原学和免疫学指标及其临床症状的关系[J].中华实验和临床病毒学杂志, 1999,13:1.
11.Hulilng Hu,et al .Infectivities of Human and Othere primateLentiviruses Are Activated by Desialylation of the Virion Surface[J].J Virol,1996, 70(11): 7462
12.Cheng-Mayer C, et al.Altered host range of HIV-1 after passagethrough various human cell types [J]Virology, 1991, 181: 288.
13.Alkhatib G et al.A RANTES, MIP-1 α, MIP-1 β receptor as a fu-sion cofactor for macrophage-tropic HIV-1 [J].Science, 1996,272:1955.
14.Feng Y, et al.HIV-1 entry cofactor: functional cDNA colning of aseven-transmenbrane, G protein coupled receptor [J] .Science,1996,272:872.
15.Speck RF, et al.Selective employment of chemokine receptor ashuman immunodeficiency virus type 1 coreceptors determined by in-dividual amino acid within the envelope V3 locp[J].J Vinol1997,71:7136.
