Abstract:In this paper,gel filtration chromatography (GFC) and ionexchange chromatography (IEC) were used to purify the rabies vaccine developed from Hamster kidney cells.The results indicated that to titer could reach to 4.8 IU/ml with GFC method a higher virus titer,in addition,fetal calf serum residue was less than 50ng/ml,which is in conformity with the requests of “ the Procedures of China Biological Products ”.However,using IEC method again after GFC resulted in a great deal of decrease of vaccine virus titer,which was 1.9IU/ml.The lost amount of the rabies viruses was great.Therefore,only the GFC used in the paper to purify the rabies vaccine developed from Hamster kidney cells is an effective,and with wiich a practicable method.Furthermore,this paper also provides a technique basis for the manufacture of vaccines on a large scale.