Abstract:Detection and analysis of rickeckettsial by monclonal antibodies to Heilonjingii 054 strain of spotted FeverGroup Rickettsiae (SFGR) with micro indirect Immunofluorescence (mllF) and primer pairs for PCR of Rickettsial were reported. The mIIF reaction of mcAb2E and mcAb4G2, were positive with all of the species of SFGRused, included R. rickettsii、 R. sibirica、 R. conorii、 R. pakeri、R. australia and R. heilongjiangii 054 strain; R. rickttsii and 054 strain were detectable with McAbF7,The results of PCR with different primer pairs for Rickettsiaerevealed that 381bp bands appeared with primers RpCS877p-1258n PCR for the followings: R. tsutsugamushi、R. prowazekii、R. mooseri and SFGR. There were 532bp and 1222bp hands with primers R190-70p-602n andRr190. 4442p-5664n for PCR of rickettsiae that were same with those rickettsiae with RpCS primres except R.tsutsugamush. Only apeared the 388bp band of R. tsutsugamushi by PCR with Rt120 and there were 523bp bandsof SFGR by PCR with Rr. 120. 2048p-1625n primer pair. The results above showed that the detection and diagnosis of rickettsiae were possible with these methods.