Abstract：In this study,the whole DNA of L.biflexa sv Patoc strain Patoc 1 was labelled with photohiotin. It was used as a probe to detect different serovars of L. interrogans,and L. biflexa sv Andamana Strain CH11 by the dot-hybridization method on a nintrocellulose membrane and the microtiter plate method.The results showed that only the DNA of L. biflexa(strain CH11 and strain Patoc 1) could be recongnized by this probe. No hybridization was found between the DNA probe of strain Patoc 1 and all the tested pathogenic leptospiral DNA of various serovars.This finding indicated that photobiotin-DNA probe of strain Patoc 1 could be used to differentiate L.biflexa from L. interrogans.
