Abstract:Two antigenic substances, i.e. leptospiral erythrocyte sensitizing sub-stance(ESS) and lipopolysaccharide(LPS), were extracted from Leptospira interrogans serovar lai strain 70091. They were incorporated onto liposomal bilayers vespectively to form new immune preparations. Then the protection test in Syrian hamsters and the detection of antibody responses in rabbits were carried out. The results revealed that DPPC:Chol:DCP liposomes could markedly enhance the immunogenicity of the ESS. The survival rate was 96.6% in the hamsters immunized with liposome-ESS whilst only 36.7% at 40 μg of the ESS per hamster by the Ess alone. The level of antibody response in rabbits was raised too. On the other hand, no protective immunity was detected in hamsters administered with the L-LPS preparations, whether it was used alone 01 carried in liposomes. However, the rabbit antiserum against the L-LPS could induce passive immunity in hamsters.
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