Abstract：To establish realtime PCR for quantitative detection of Salmonella spp., a pair of primers and fluorescent probe were designed according to stn gene sequence of Salmonella spp., and then a realtime PCR method was successfully established to detect Salmonella spp. through the exploration of reaction system and reaction conditions. Results demonstrated that the method is specific and sensitive. Salmonella spp. detected by this realtime PCR was positive while nonSalmonella one was negative. The correlation coefficient of standard curve (R2) was 0.993 and the detecting threshold was up to 5 CFU. After the detection of 108 chicken feces, 50 chicken meats and 58 water samples were detected with the this method and the positive rates were 3.7% (6/108), 4% (2/50) and 3.4% (2/58) respectively. The result was consistent with results from traditional microbiology method. The method is simple, rapid, specific and sensitive, and this study made a good foundation for rapid detection of Salmonella spp. in the environment study or disease diagnosis.
2010, Vol. 26 
