Detection and expression of recombinant protein PrP102-242 in COS-1 cells
Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences,
State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Public Health
of Ministry of Agriculture, Key Laboratory of Animal Virology of Ministry of Agriculture, Lanzhou 730046, China
Abstract:To detect the expression of product of recombinant protein PrP102242 in COS1 cells, purified recombinant protein of PrP102242 were transfected into COS1 cells, to obtain stable cell lines using different concentration of G418. After that, the expression of recombinant protein PrP102242 in COS1 were detected by using indirect immunity fluorescence assay(inIFA), indirect enzyme linked immunosorbent assay (inELISA) and Western blot assay. The result showed that expression of pCIp264 in COS1 were detected by using inELISA, IFA and Western blot.
丁耀忠, 刘永生, 陈浩泰, 刘文倩, 张杰. 重组PrP102-242在COS-1中的表达及表达产物的检测[J]. 中国人兽共患病学报, 2010, 26(12): 1097-1100.
DING Yao-Zhong, LIU Yong-Sheng, CHEN Hao-Tai, LIU Wen-Qian, ZHANG Jie. Detection and expression of recombinant protein PrP102-242 in COS-1 cells. Chinese Journal of Zoonoses, 2010, 26(12): 1097-1100.
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