Abstract:In order to clone, express and identify the antigenicity of the outer membrane protein rOMP18 of Campylobacter jejuni (C. jejuni), omp18 was amplified by PCR and ligated to plasmid pET32a(+). After the recombinant plasmid was transformed to E.coliBL21 (DE3),the expression was induced by IPTG. The expressed protein was identified with SDSPAGE and its antigenicity was determined by Western blot (WB) analysis. Restriction enzyme analysis showed the recombinant plasmid pET32aomp18 have been successfully constructed. The recombinant expressed protein had a weight of 34kD(HIS16kD), which was similar to the predicted histag and the protein rOMP18. WB result indicated the expressed rOMP18 had good antigenicity which inferred it might be a potential antigen for the serological diagnosis of C. jejuni infection.
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