Abstract：To construct and express CsGST2 CsTP22.3 fused protein of Clonorchis sinensis，and evaluate its applicability in serological diagnosis of clonorchiasis, CsGST2 and CsTP22.3 genes were successively cloned into the prokaryotic expression vector pET32a ( + ) and then expressed in E. coli BL21 by IPTG induction. Then the recombinant CsGST2 CsTP22.3 fusion protein was expressed and identified by SDSPAGE and Western Blotting. PCR, double enzyme digestion and DNA sequencing confirmed that the recombinant expression plasmid was successfully constructed. The recombinant fusion protein could elicit efficiently specific antibodies in SD rats, indicating its ability of immunogenicity and laying the foundation for further study of its function.
李彩霞, 王慧玲, 胡旭初, 余新炳. 华支睾吸虫GST2TP22.3融合蛋白的构建及其免疫原性初步研究[J]. 中国人兽共患病学报, 2011, 27(1): 49-52.
LI Cai-Xia, WANG Hui-Ling, HU Xu-Chu, YU Xin-Bing. Preparation of fusion protein GST2TP22.3 of Clonorchis Sinensis and analysis of immunogenicity of the recombinant protein. Chinese Journal of Zoonoses, 2011, 27(1): 49-52.
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2011, Vol. 27 
