rSj26GST-Sj32融合蛋白-IgG4-ELISA诊断慢性血吸虫病的研究

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摘要目的探讨rSj26GSTSj32IgG4ELISA诊断慢性血吸虫病的价值。方法用rSj26GSTSj32融合蛋白作为包被抗原，通过rSj26GSTSj32IgG4ELISA检测慢性日本血吸虫病患者血清抗体，同时以华支睾吸虫病、卫氏并殖吸虫病、泡型棘球蚴病、囊型棘球蚴病、乙型肝炎、肺结核患者血清和健康人血清作为对照。结果该法检测慢性血吸虫病的敏感性和特异性分别为95.00%和100.00%，且与泡型棘球蚴病、囊型棘球蚴病、乙型肝炎和肺结核患者血清均无交叉反应；诊断慢性血吸虫病的阳性预告值、阴性预告值及诊断效率分别为100.00%、95.56%和97.59%。结论rSj26GSTSj32IgG4ELISA可用于慢性血吸虫病的免疫诊断。

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	蔡世飞
	李文桂
	王敏

关键词 ：关键词：ELISA, rSj26GSTSj32融合蛋白, SjAWA, IgG4

Abstract：In order to study the value of rSj26GSTSj32IgG4ELISA for diagnosis of chronic schistosomiasis, the IgG4 antibodies were observe to detect sera of chronic schistosomiasis with rSj26GSTSj32 fusion protein by rSj26GSTSj32IgG4ELISA and the sera of patients with clonorchiasis sinensis, paragonimiasis westermani, alveolar echinococcosis, cystic echinococcosis, hepatitis B and pulmonary tuberculosis as well as the healthy people were taken as controls. The sensitivity and specificity of this method were 95.00% and 100.00% respectively, and there was no cross reaction with the sera of patients with alveolar echinococcosis, cystic echinococcosis, hepatitis B and pulmonary tuberculosis. The positive predictive value, negative predictive value and efficiency of diagnosis were 100.00%, 95.56% and 97.59%, respectively. It is suggested that rSj26GSTSj32IgG4ELISA could be applied to immunodiagnosis for chronic schistosomiasis.

Key words： ELISA recombinant Sj26GST-Sj32 fusion protein SjAWA IgG4

PACS:

R532.2

基金资助:

重庆市科委地方病重大专项基金（No.2008AB5055，2008AB5008，2008AB5054）资助

通讯作者: 李文桂，Email:Li_wengui@yahoo.com.cn

引用本文:

蔡世飞, 李文桂, 王敏. rSj26GST-Sj32融合蛋白-IgG4-ELISA诊断慢性血吸虫病的研究[J]. 中国人兽共患病学报, 2011, 27(3): 212-216. CAI Shi-Fei, LI Wen-Gui, WANG Min. Diagnosis of chronic schistosomiasis with recombinant Sj26GSTSj32 fusion protein by ELISA. Chinese Journal of Zoonoses, 2011, 27(3): 212-216.

链接本文:

http://www.rsghb.cn/CN/ 或 http://www.rsghb.cn/CN/Y2011/V27/I3/212

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