Abstract:An atypical enteropathogenic Escherichia coli (aEPEC) strain, firstly documented in Fujian, was isolated from a fecal specimen of a fatal infant case in October, 2010. The purpose of this study was to investigate characterization of this pathogenic strain, facilitating further research on aEPEC. A variety of assays were performed, including routine systematic biochemical identification, serotyping, antibiotics sensitivity tests, PCR amplification of virulent genes, and sequence analysis of eaeA gene. Biochemical characterization indicated this isolate was Escherichia coli, not agglutinating with typical EPEC antisera available. Antibiotic resistance tests showed susceptible to most drugs; PCR detection of virulent genes revealed eaeA+, bfp, stx1, stx2, ehxA, paa;BLAST results of partial sequenced eaeA gene indicated identical to the eae gene for intimin of Escherichia coli EHEC and EPEC. Therefore, routine biochemical assays, molecular diagnosis and sequence analysis confirmed this strain was the first identified aEPEC isolate in Fujian, and extensive investigation should be conducted to survey its virulenceassociated genes and address virulent markers of aEPEC isolates.
陈爱平, 陈建辉, 杨劲松, 林杰, 郑金凤, 严延生. 福建省非典型肠致病性大肠杆菌(aEPEC)的发现及某些分子特征的研究[J]. 中国人兽共患病学报, 2011, 27(4): 275-278.
CHEN Ai-Ping, CHEN Jian-Hui, YANG Jin-Song, LIN Jie, ZHENG Jin-Feng, YAN Yan-Sheng. Identification and preliminary molecular characterization of an atypical enteropathogenic Escherichia coli strain from Fujian. Chinese Journal of Zoonoses, 2011, 27(4): 275-278.
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