Abstract:To establish rapid detection method of enterohemorrhagic E. coli O157∶H7 by using
immunomagnetic separation (IMS) and realtime PCR, a pair of primers and TaqMan probe were
designed based on the rfbEO157 gene of E. coli O157∶H7. E. coli O157∶H7 was captured by
the O157 immunomagnetic beads from bacterial suspensions, and then assayed by the realtime
PCR. The results of detection were positive for the samples containing E. coli O157∶H7, but
negative for samples containing other bacteria. The lowest detection limit of immunomagnetic
separation and realtime PCR was 3.3×102 CFU per milliliter. It just took 4 hours to get
the results. It is concluded that the immunomagnetic separation and realtime PCR for
detected E. coli O157∶H7 in specimen shows high specificity, sensitivity, simplicity and
celerity. Therefore, the method could be used to detect E. coli O157∶H7 quickly and
identify the causes of outbreak.
王涛, 刘凯, 王艳, 綦廷娜, 叶长芸, 熊衍文. 应用免疫磁珠分离法及荧光定量 PCR技术快速检测肠出血性大肠埃希菌O157∶H7[J]. 中国人兽共患病学报, 2011, 27(4): 291-293.
WANG Tao, LIU Kai, WANG Yan, QI Ting-Na, YE Chang-Yun, XIONG Yan-Wen. Rapid detection of Escherichia coli O157∶H7 by immunomagnetic separation and realtime PCR. Chinese Journal of Zoonoses, 2011, 27(4): 291-293.
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