红霉素和克林霉素对穿通支原体诱导膀胱移行细胞癌干预作用的实验研究

摘要
图/表
参考文献
相关文章 (15)

全文: PDF (3950 KB) HTML (0 KB)
输出: BibTeX | EndNote (RIS)

摘要目的试图探讨抗生素对穿通支原体（Mycoplasma penetrans，Mpe)诱导免疫缺陷小鼠膀胱移行上皮细胞癌的干预作用，为临床上免疫缺陷患者协同穿通支原体感染诱导膀胱肿瘤的干预提供实验依据。方法 70只ICR小鼠隔日腹腔注射环磷酰胺制备免疫抑制模型，分红霉素、克林霉素干预试验组（n=20）在上行注射0.1mLMpe（6×1089 /mL）的同时肌肉注射红霉素（30mg/kg·d）或克林霉素（25mg/kg·d），连续保护4d。Mpe非干预对照组（n=20）上行感染相同菌量Mpe，NS对照组（n=10）上行注射相同剂量的NS，实验组在第4，8，18w分批宰杀，取小鼠膀胱组织进行微生物学和超微结构检查，计算各组血清培养菌落数，比较显著性差异，并观察小鼠膀胱超微结构的形态学改变。结果红霉素和克林霉素干预组小鼠菌落检出率较非干预对照组均有显著降低(P <0.01);电镜结果显示红霉素和克林霉素干预试验组小鼠的膀胱组织超微结构显著改善。结论红霉素和克林霉素对Mpe诱导膀胱移行上皮细胞癌的发生有干预作用。

	服务

	把本文推荐给朋友
	加入我的书架
	加入引用管理器
	E-mail Alert
	RSS
	作者相关文章
	任帆
	周丽萍
	王伟华
	闫涛
	方周溪

关键词 ：穿通支原体, 动物模型, 膀胱移行上皮细胞癌, 免疫缺陷

Abstract：The aim of the present study was to investigate the antimicrobial intervention of erythromycin and clindamycin against urinary bladder transitional epithelium cell cancer induced by Mycoplasma penetrans(Mpe) in immunedeficient mice, so as to provide the experimental evidence in intervention of the bladder cancer in immunedeficient patients, who has infection caused by Mycoplasma penetrans. All 70 ICR was render to be immunosuppressive by intraperitoneal injections of cyclophosphamide(50mg/kg d) every other day. The erythromycinintervention group and clindamycinintervention group (n=20) were firstly received 0.1ml Mpe(6×108-9/mL) by ascending infection through urethra, then received erythromycin (30mg/kg d) and clindamycin(25mg/kg d) respectively by intramuscular injection for continuous 4 days. Nonintervention group (n=20) received equal Mpe, and normal saline contrast group(n=10) received equal strokephysiological saline solution respectively. The treated mice were killed 4, 8 and 18 weeks later and the bladder tissues were taken for microbiological cells and ultrastructure examination. The number of Mpe cultured in serum in each group was evaluated and compared. The morphological changes of the bladder migratory cells were observed. The detection rate of colony in the intervention groups and nonintervention groups had significant difference (P<0.01). Ultrastructural observation showed the tissue structures of the bladder in intervened mice were improved than those without intervention. It’s indicated that the erythromycin and clindamycin could intervene immunedeficient mice against the bladder cancer induced by Mycoplasma penetrans.

Key words： Mycoplasma penetrans（Mpe） animal models bladder transitional epithelium cell cancer immunedeficient

PACS:

R375

通讯作者: 周丽萍，Email: zlpzlp19491949@163.com

引用本文:

任帆, 周丽萍, 王伟华, 闫涛, 方周溪. 红霉素和克林霉素对穿通支原体诱导膀胱移行细胞癌干预作用的实验研究[J]. 中国人兽共患病学报, 2011, 27(4): 336-339. REN Fan, ZHOU Li-Ping, WANG Wei-Hua, YAN Tao, FANG Zhou-Xi. Intervention effect of erythromycin and clindamycin on the bladder transitional epithelium cell cancer induced by Mycoplasma penetrans. Chinese Journal of Zoonoses, 2011, 27(4): 336-339.

链接本文:

http://www.rsghb.cn/CN/ 或 http://www.rsghb.cn/CN/Y2011/V27/I4/336

\[1\]Brenner C，Neyrolles O，Blanchard A．Mycoplasmas and HIV infection：from epidemiology to their interaction with immune cells\[J\]．Front Biosci，1996，1：42 54． \[2\]Tsai S，Wear DJ．Shih JW ，et al．Mycoplasmas and oncogenesis：Persist infections and multistage malignant transformation\[J\]．Proc Natl Acad Sci USA，1995，92(22)：1019710201 ． \[3\]Feng SH ，Tsai S，Rodriguez，et al．Mycoplamal infections prevent apoptosis and induce malignant transformation of interleukin3dependent 32D hematopoietic cells\[J\]．Mol Cell Biol，1999，19(12)：79958002． \[4\]Polianskaia GG，Efremova TN ，Sakuta GA.Effect of Mycoplasma eontamination of human cervical carcinoma cell line M Hela clone 11 on karyotypic variability\[J\]．Tsitologiia， 2000，42（8）：794801． \[5\]周丽萍，余志贤，蓝升红，等．肿瘤患者血液和肿瘤组织中穿通支原体的分离与鉴定及其电镜观察 \[J\]．中华医学杂志，2006，86（26）：18621864． \[6\]朱燕英，周丽萍，张乾，等．穿通支原体在宫颈癌患者血液和肿瘤组织中的分离检出\[J\]．中国人兽共患病学报，2007，23（6）：537543． \[7\]Zhou Liping，Wen Xiushu，Zhu Yanying，et al．Mycoplasma penetrans were isolated from blood and tumor tissue of cancer patients\[J\]．U．S Chinese Journal of Iymphology and Oncology，2007，6(3)：129136． \[8\]Poulin SA, Perkins RE, Kundsin RB. Antibiotic Susceptibilities of AIDSAssociated Mycoplasmas\[J\]. J Clin Microbiol, 1994,32（4）：11011103. \[9\]Hayes MM, Wear DJ, Lo SC. In vitro antimicrobial susceptibility testing for the newly identified AIDSassociated Mycoplasma, Mycoplasma fermentans(incognitus strain)\[J\]. Arch Pathol Lab Med, 1991, 115（5）:464466. \[10\]Yáez A, Cedillo L, Neyrolles O, et al. Mycoplasma penetrans bacteremia and primary antiphospholipid syndrome \[J\]. Emerg Infect Dis,1999,5（1）:1647. \[11\]Lo SC, Hayes MM, Tully JG, et al. Mycoplasma penetrans sp.nov.,from the urogenital tract of patients with AIDS \[J\]. Int J Syst Bacteriol,1992,42（3）:357364. \[12\]Feng SH, Tsai S, Rodriguez J. Mycoplasmal infections prevent apoptosis and induce malignant transformation of interleukin3dependent 32D hematopoietic cells \[J\]. Mol Cell Biol,1999,19(12):79958002. \[13\]Zhou LP, Yu ZX, Lan SH, et al. Isolate culture and being observed by electron microscope of Mycoplasma penetrans of blood and adjacent tissues from cancer patients \[J\]. National Medical Journal of China, 2006, 86 (26):18621864. \[14\]Hayes MM, Foo HH, Timenetsky J, et al. In vitro antibiotic susceptibility testing of clinical isolates of Mycoplasma penetrans from patients with AIDS \[J\]. Antimicrob Agents Chemother,1995,39(6):13861387. \[15\]Hayes MM, Foo HH, Kotani H, et al. In vitro antibiotic susceptibility testing of different strains of Mycoplasma fermentans isolated from a variety of sources \[J\]. Antimicrob Agents Chemother,1993,37(11):25002503.