Abstract:Abstract Object To identify and detect Dermatophagoides farina (D.f) and Dermatophagoides pteronyssinu(D.p)by Polymerase Chain Reaction (PCR) technology. Methods A randomly primed PCR(RP-PCR) technology were used to amplify the DNA of D,f and D.p, three amplified bands were selected for DNA sequencing and analysis. According to the results of DNA sequencing, another two pair of primers were designed to identify and detect D,f and D.p, then the specificity and sensitivity of these primers analyzed. Results In amplification of RP-PCR, two significant bands around 500bp and 750bp were seen in amplified products of D.f; while only a clear band about 500bp in D.p and no product larger than 500bp appeared. Three new DNA fragments of D,f and D.p were sequenced and analyzed in homology. The new primers designed are highly specific and sensitive in detection of the two mites. Conclusions RP-PCR technology can be used to identify D,f and D.p. The PCR method verified in the research have a potentiality to detect D,f and D.p in the environment.