Abstract:In order to investigate the distribution of Paragonimus in northern suburban of
Guangzhou, snails and crabs were collected from mountain streams and the cercariae and
metacercariae of Paragonimus were detected and dissected. Cats and dogs were artificially
infected with the metacercariae of Paragonimus from infected crabs, as results, the adult
worms of Paragonimus were detected from these cats and dogs. The COI and ITS2 sequence
analysis were performed in the adult worm samples, and the sequence results were compared
with those of Paragonimus sequence in GenBank. It was found that for snail samples in
Liangkou, Nankun Mountain and Lvtian areas, the average infection rates of cercariae were
0.32% (4/1,210), 0.15% (3/2,000) and 0.03% (1/310) .The snail species in both Liangkou and
Nankun Mountain areas were identified as Semisulcospira libertine, while the snail species
in Lvtian area was Tricula.;for crab samples in Liangkou area, the average infection rate of
P. westermani metacercariae was 100% (35/35), and the crab species were identified as
Sinopotamon pinheense. The average numbers of the metacercariae in per crab and per gram of
crab were 79.4 and 11.12, respectively .The highest numbers of metacercariae in one crab was
1 050. And for crab samples in Nankun Mountain area, the average infection rate of P.
westermani metacercariae was also 100% (59/59), and the crab species were also identified as
Sinopotamon pinheense. The
average numbers of the metacercariae in per crab and per gram of crab were 105.66 and 7.87,
respectively. For crab samples in Lvtian area, the average infection rate of Pagumogonimus
skrjabini was 36.73% (36/98), and the crab species were identified as Nanhaipotomon
pinyuanense. The average numbers of the metacercariae in per crab and per gram of crab were
4.55 and 0.53, respectively. The DNA sequences for COI and ITS2 from the adult worms of P.
westermani from the Liangkou
and Nankun Mountain areas detected 99%, 98% and 98%, 97% homologous with those of P.
westermani in the GenBank DNA database. There are two ultrahigh infectious focus of P.
westermani found in northern suburban Guangzhou, and one moderate infectious focus of
Pagumogonimus skrjabini.
陆予云, 沈浩贤, 邝浩成, 刘巧, 赵太平, 龙小山, 罗健留, 冼少龙, 傅广华, 乔亚峰. 广州北部山区并殖吸虫流行分布现状初步研究[J]. 中国人兽共患病学报, 2011, 27(7): 605-609.
LU Yu-Yun, SHEN Hao-Xian, KUANG Hao-Cheng, LIU Qiao, ZHAO Tai-Ping, LONG Xiao-Shan, LUO Jian-Liu, XIAN Shao-Long, FU Guang-Hua, QIAO Ya-Feng. Epidemic distribution of Paragonimus in northern suburban of Guangzhou. Chinese Journal of Zoonoses, 2011, 27(7): 605-609.