Abstract：ABSTRACT: To pack the recombinant adenovirus vector carrying SV40LT gene and to study the effect of the recombinant adenovirus on transfecting schistosomulum cells. The constructed recombinant adenovirus plasmid was re-transformed into competent cells Stbl2, then identified by restriction enzyme digestion. The right recombinant adenovirus plasmid was transfected into 293A cells using Lipofectine 2000. The recombinant adenovirus was amplified, and then identified by polymerase chain reaction (PCR). The effect of the recombinant adenovirus transfecting schistosomulum cells was detected by RT-PCR and immunohistochemistry. The recombinant adenovirus containing SV40LT gene was successfully packed, which effectively infected schistosomulum cells. The expression of SV40LT gene in schistosomulum cells was detected by RT-PCR and immunohistochemistry. The recombinant adenovirus containing SV40LT gene was successfully packed and could transfect schistosomulum cells to provide a basis for Schistosoma japonicum cells immortalization.
2011, Vol. 27 
