Abstract:With the rapid development of molecular biology, molecular systematics has been developing dramatically, which had made progress in the classification of species and been paid more and more attention. Molecular classification technology represents for a new method to aid in the identification of species, which can not only distinguish the closely related and cryptic species effectively, but also shows broad application prospect in the study of molecular classification technology in terms of population evolution,origins and phylogenetic relationships of species. In the present paper, the progress on molecular classification of biting midges were summarized and the application of several commonly marker genes were introduced.