Abstract:ABSTRACT:In order to survey the epidemic focus of paragonimiasis in Shen Nong-jia and provide the basis for drawing up adequate control strategy. We carried out an expending experiment in the serum antibody of the crowd in the popular area was detected by DIGFA-kit and compared with the result by ELISA.The infection situation of intermediate host and reservoir host in the popular area was investigated simultaneously. Results showed that the total of 1128 serum samples collected from the crowd, 76 were positive with the positive rate of 6.74%.The masculine coincidence rate was 98.7% by ELISA. The infection rate of snail considered as the first intermediate host was 0.91%, the infectiosity of limnetic crab considered as the second intermediate host was 3.21 larvae per crab, and the natural infection rate of cat and civet cat was 51.72% and 15.79%. Thus,the DIGFA-kit has good sensitiveness and peculiarity , and is suitable for in the investigation in clinical checkout and largescale epidemiology in popular area. The existence of the first and the second intermediate host and the reservoir host in the inquired area suggests the that area is a paragonimiasis skrjabini popular area.