Abstract：The aim of the current study is to investigate the population genetic structure of Echinococcus granulosus(E. granulosus) obtained from Qinghai-Tibetan Plateau, and provide the foundational data for prevention and control of echinococcal disease. The complete sequences of mitochondrial DNA 12S genes of 98 isolates which came from different areas (including Qinghai, Tibet and Sichuan populations) and different hosts (such as human being, sheep, or cattle) were sequenced ，and combined with forty-two 12S sequences of Qinghai sheep strains which had reported analyzed. The result of sequences analysis was that all 56 isolates were identified to be E. granulosus sensu stricto (genotype G1-G3). Of 98 strains were classified into 13 haplotypes (ES1-ES13), while 15 nucleotide variation sites were detected. Haplotypes diversity and nucleotide diversity were 0.381 and 0.00092, respectively. The nucleotide diversity (0.001353) and genetic distance (0.00135) of Sichuan population were the highest one in the three populations. It was showed by AMOVA that 97.36% of the total genetic variability was found among haplotype groups. NJ tree and Mrbayes tree showed that ES10 and ES11 formed a haplotype group which was different from other haplotypes. Network showed that ES1 was the main haplotype, and others centered to it, with no geographic cluster finding. The points of Nm and FST showed that there was not significant genetic differentiation among populations. The result showed that E. granulosus of genotype G1-G3 were found in Qinghai-Tibetan Plateau. Genetic diversity existed in and among the populations of Qinghai-Tibetan Plateau, and the genetic diversity of Sichuan population was higher than that of other populations. The variation which happened in the population leads to the intraspecific variation. Geography cluster has not been found, and the genetic differentiation is not significant
2013, Vol. 29 
