Abstract：To investigate the HIV-1 drug resistant gene mutation rate on protease and reverse transcriptase in HIV infected MSM population without HAART in Fujian province in order to provide evidences for selection of appropriate ARV drugs. HIV-1 viral RNA were extracted from plasma samples and then amplified the whole pol gene and partial reverse transcriptase gene by reverse transcription polymerase chain reaction (RT-PCR)．The sequences of those amplified fragments were edited using the software Contig Express, and then submitted to the website http://hivdb.stanford.edu/ to analyze their drug resistance status. Meanwhile, their phylogenesis was analyzed by Bio-Edit, Clustal and Mega software. It was found that 60% (30/50) of that strains were classified as CRF01_AE, 10 were CRF 07_BC, 9 were B and one was CRF 08_BC. According to the interpretation of Stanford university drug resistance database, 5 of 50 sequences occurred mutations for PIs, only one with M46I mutation was interpreted as medium lever resistant to NFV. 10 sequences occurred mutations for non-nucleoside reverse transcriptase inhibitors (NNRTIs) and nucleoside reverse transcriptase inhibitors (NRTIs) and the one carried V179E was interpreted as potential low lever resistance to NNRTIs. These results reveals that although the low mutation rate was showed in MSM HIV-1 infected patients in Fujian province, enhancing monitoring the transmission of drug-resistant HIV-1 in this population is necessary.
2013, Vol. 29 
