Abstract:ABSTRACT: In order to analyze the protein structure of Chlamydophila pneumoniae Cpn0797 protein and to prepare specific monoclonal antibodies(mAb) against Cpn0797 protein. ProtParam, SignalP, NPS@ and PSORT software packages were used to predict the physical and chemical properties, signal peptide, secondary structure and protein localization sites in cells according to the amino acid sequence of Cpn0797. The gene cpn0797 was cloned in the prokaryotic expression vector pGEX6p-2, expressed in E. coli and purified. mAbs against Cpn0797 were prepared with the hybridoma technique after mice were immunized with the purified GST-Cpn0797 fusion protein. The isotype and specificities of the mAbs were determined by an indirect immunofluorescence assay (IFA). The bioinformatical analysis results showed that Cpn0797 protein consists mainly of random coils. Hybridoma cell lines stably secreting mAbs against Cpn0797 protein were obtained. The mAbs reacted with Cpn0797 endogenous protein. In conclusion, the specific mAbs against Cpn0797 protein were obtained, which provides a basis for further studying the biological function of Cpn0797 protein.