Abstract:To construct the recombinant Bb-hpaA-vacA vaccine of Helicobacter pylori(Hp). hpaA and vacA antigen gene were amplified by PCR and the fusion gene hpaA-vacA was obtained with gene SOEing. Then the fusion gene was cloned into Escherichia coli-Bifidobacteria shuttle plasmid pGEX-1λT to construct pGEX-hpaA-vacA. The recombinant plasmid was electroporated into Bifidobacteria bifidum(Bb) to construct rBb hpaA-vacA vaccine. A 1500bp fusion gene of hpaA-vacA was successfully amplified by PCR and cloned into pGEX-1λT by restriction analysis, and the rBb hpaA-vacA vaccine was successfully constructed by PCR and restriction analysis. In this way, the rBb hpaA-vacA vaccine of Helicobacter pylori is successfully constructed, which lays the experimental foundation of exploitation and utilization of this vaccine.