Abstract:AIM:To investigate the effect and mechanism of Resolvin E1 on liver fibrosis mice models with schistosomiasis japonica by detecting the changes of serum levels of cytokines and liver-fibrosis.
METHODS:Mice were infected with schistosoma japonicum cercariae percutaneously, to construct mice models of liver fibrosis of schistosomiasis japonica. The infected mice were divided randomly into three groups at the end of the 5th week after infection:model group, model control group (to inject normal saline), treated group (to inject RvE1) and normal control group. The mice were killed at the end of the 10th week. The serum level of collagen IV (C-IV),precollagen Ⅲ (PCⅢ), hyaluronic acid (HA) and laminin (LN) were evaluated by radioimmunoassay (RIA). The serum level of TNF-α and IFN-γ were evaluated by ELISA.
RESULTS:After infection 10wk, no mice naturally died, and the weight of mice was no significant changes in each group. The serum level of liver fibrosis index in RvE1 treated mice,including the collagen IV (C-IV),precollagen Ⅲ (PCⅢ), hyaluronic acid (HA) and laminin (LN), were significantly lower than with the model control group, and there was a significant difference (P <0.05). The serum level of TNF-α in RvE1 treated mice, was significantly lower than with the model control group, and there was a significant difference (P <0.05). The serum level of IFN-γ in RvE1 treated mice, was significantly high than with the model control group, and there was a significant difference (P <0.05).