Abstract：To evaluate the applicability of multiple-locus variable-number tandem repeat analysis method for Legionella subtyping, 9 VNTR locus were selected to type 81 Legionella pneumophila serogroup 1 strains isolated from environmental water in China. There were 6, 3, 4, 2, 3, 3, 2, 4 and 7 different products amplified from 9 VNTR locus respectively. No specific amplification was got from several locus of some strains. Repeated sequences were identified in PCR products of 7 locus. No repeated sequences were found in all sequenced products from loci Lpms3 and in some products from loci Lpms31. The sequences of tandem repeats varied within isolate and among isolates. Incomplete tandem repeats were found in 3 locus. Based on the polymorphisms of 8 locus, 81 Lp1 strains could be differentiated into 19 types. Comparatively, these strains could be divided into 46 and 21 molecular types by PFGE and SBT methods respectively. So, MLVA method was less sensitive for Legionella subtyping. But it was simple, cheap and could be used in relatively simple equipped laboratories.
2012, Vol. 28 
