Abstract:Abstract:【Objective】To construct eukaryotic expression pCDNA-ROP5 and evaluated the immune responses it induced in Kunming mice.【Methods】ROP5 gene fragment was amplified by PCR and insert into pCDNA-3.1 vector to form the recombinant plasmid pCDNA-ROP5;Hela cells were transfected by plasmid pCDNA-ROP5 with lipofection strategy;plasmid pCDNA-ROP5, pCDNA-3.1 and NS were injected into Kunming mice muscle three times, antibody IgG level were measured in each group; invested the survival time after challenged by T. gondi【Results】The results showed that the ROP5 has been successfully expressed in Hela cells and developed a high level of specific antibody response, Survival time of ROP5 immune mice was prolonged when infected by T.gondii.【Conclusion】ROP5 could trigger immmuno-protection against T. gondii challenge.
收稿日期: 2012-01-20
出版日期: 2013-06-17
基金资助:海南省自然科学基金“弓形虫MIC8真核表达载体构建及免疫原性研究”
通讯作者:
赵焕阁
E-mail: gezi8004@163.com
引用本文:
赵焕阁. 弓形虫ROP5基因真核表达载体的构建及其免疫保护性的研究[J]. 中国人兽共患病学报, 2012, 28(9): 947-950.
Zhao Huange. Construction of eukaryotic expression vector of Toxoplasma gondii ROP5 gene and its immunoprotective effect. Chinese Journal of Zoonoses, 2012, 28(9): 947-950.