Abstract:Objective To establish a method of Loop-mediated isothermal Amplification (LAMP) for detection of Campylobacter jejuni. Methods According to Campylobacter jejuni gyrA gene sequences published on GenBank, we designed specific loop-mediated isothermal amplification primers and developed a novel and highly specific loop-mediated isothermal amplification assay for the sensitive and rapid detection of Campylobacter jejuni. Their Campylobacter jejuni natural infection rates were tested in ten chicken breeds. Results The products of LAMP demonstrated the typical ladder patterns and digested fragments were found with the predicted sizes. Sensitivity of the LAMP asssay for direct detection of Campylobacter jejuni in pure cultures was 20cfu/mL. It was 10-fold more sensitive than that of the conventional PCR assay. The assay correctly identified Campylobacter jejuni, but did not detect 7 non-Campylobacter jejuni strains. The Campylobacter jejuni natural infection rate was 6%-90% in the ten chicken breeds. Conclusion The LAMP assay is a sensitive, rapid and simple tool for the detection of Campylobacter jejuni and will play a role in clinical detection.