Abstract：Abstract: In this study, we confirmed the first case of human infection with influenza A (H7N9) virus in Fujian province by viral specific nuclear acid detection and sequencing the four segments of viral genome. The respiratory specimen (throat swab) was collected from an pneumonia with unexplained origin case reported in Longyan city, Fujian province. Viral RNA was extracted form the specimen and applied for real-time reverse transcript PCR with universal primer/probe sets for influenza A & B, specific primer/probe sets for seasonal influenza A virus (including H3N2 subtype, H1N1 subtype and H1N1pdm), specific primer/probe sets for newly emerging H7N9 virus, respectively. The four near full-length segments of viral genome were sequenced by using two-step RT-PCR methods with a set of primers designed by ourselves. Positive results obtained with universal influenza A virus and specific H7N9 subtype virus primer/probe sets, whereas negative results with the other primer/probe sets. Four segments of viral genomic sequence were obtained and compared with the published N7N9 viral genomic sequences. The results of real-time RT-PCR and viral segments sequencing revealed that presence of H7N9 subtype influenza A virus in the specimen from the pneumonia with unexplained origin case. Viral sequence was highly identical to that of H7N9 subtype viruses currently epidemic in mainland China. It should conclude that the patient was the first case of human infection with novel H7N9 subtype virus in Fujian province.
2013, Vol. 29 
