Abstract： The aim of this study was to identificate 2 strains of Streptococcus suis isolated in Changsha City and elucidate their phylogenetic relationship through the 16S rRNA.The 16S rRNA of 2 strains was amplified by polymerase chain reaction (PCR) through universal primer of 16S rRNA. The products of 16S rRNA of 2 strains were obtained successfully with length about 1 500 bp. The products were sequenced successfully then the sequences were submitted to GenBank.And the sequences were compared with relational sequence through online Blast in NCBI, showeing the 2 strains of Streptococcus suis were Streptococcus suis type 2.The identification result through the 16S rRNA was consistent with traditional method.Then the phylogenetic tree of 16S rRNA was constructed through the Mege4.0 software.The phylogenetic tree showed that the 2 strains of Streptococcus suis were at the same evolutionary branching with most of Streptococcus suis type 2 and had the nearest phylogenetic relationship with Streptococcus suis 05ZYH33 in Sichuan Province.In conclusion,Streptococcus suis could be fast and accurat idendificated through the 16S rRNA and this method could be applied at rapid detection.The 2 strains of Streptococcus suis isolated in Changsha City were Streptococcus suis type 2 and were no variable in 16S rRNA gene.
收稿日期: 2012-08-18
出版日期: 2013-06-20
通讯作者:
苏良
E-mail: cscdchiv@126.com
引用本文:
苏良 欧新华. 猪链球菌长沙分离株的鉴定与16S rRNA系统进化分析[J]. 中国人兽共患病学报, 2013, 29(6): 547-550.
Liang SU Xin-Hua OU. Identification and 16S rRNA phylogenetic analysis of 2 Streptococcus suis strains isolated in Changsha City. Chinese Journal of Zoonoses, 2013, 29(6): 547-550.
2013, Vol. 29 
