Abstract：Abstract: In order to explore the variation and evolutionary status of Streptococcus suis(S. suis ) serotype 2 isolated in Hunan province,So,gene sequences and evolution relationship of cps2J、gdh and ef genes of HNCS201001、HNCS201101、HNCS201102 of human derived S. suis serotype 2 strains were analyzed. PCR primers of cps2J、gdh and ef genes of S. suis serotype 2 were designed, and the genes of HNCS201001、HNCS201101、HNCS201102 were amplifyed and sequenced,then the nucleotide sequences and the deduced amino acid sequences were analyzed with the Lasergene and Mega5 software.Analysis of the nucleotide sequences of the cps2J、gdh、ef gene revealed that the length of open reading frames(ORF) were 999 bp, 1 347 bp and 2 532 bp, and codes for proteins of 333、448 and 843 amino acid, respectively.Sequence similarity searches by using the BLAST network service showed HNCS201001, HNCS201101and HNCS201102 strains belonging S. suis serotype 2 group, and the nucleotide homology of cps2J、gdh and ef genes among the 3 strains were all 100%; Phylogenetic analysis revealed that the 3 strains in this study and the S. suis serotype 2 strains derived from pigs and human isolate in mainland China and abroad belonged to the same branch, The cps2J,gdh and ef gene of the 3 strains of S. suis serotype 2 isolated In order to explore the variation and evolutionary status of Streptococcus suis serotype 2 (S. suis 2）isolated in Hunan Province, gene sequences and evolution relationship of cps2J, gdh and ef genes of HNCS201001, HNCS201101, and HNCS201102 of human derived S. suis 2 strains were analyzed. PCR primers of cps2J, gdh and ef genes of S. suis 2 were designed, and the genes of HNCS201001, HNCS201101, and HNCS201102 were amplified and sequenced. Then, the nucleotide sequences and the deduced amino acid sequences were analyzed with the Lasergene and Mega 5 software. Analysis of the nucleotide sequences of the cps2J, gdh and ef genes revealed that the length of open reading frames (ORF) were 999 bp, 1 347 bp, and 2 532 bp, coding for 333, 448 and 843 amino acid, respectively. Sequence similarity searches by using the BLAST network service showed HNCS201001, HNCS201101 and HNCS201102 strains belonged to S. suis 2 group, and the nucleotide homology of cps2J, gdh and ef genes among the 3 strains were all 100%; phylogenetic analysis revealed that the 3 strains in this study and the S. suis 2 strains derived from pigs and human isolated in mainland China and abroad belonged to the same branch. The cps2J, gdh and ef genes of the 3 strains of S. suis 2 isolated in Hunan Province had high homology with other S. suis strains isolated in mainland China and abroad (99.8%~100% for cps2J gene, 96.4%~100% for gdh gene, and 99.7%~100% for ef gene at the nucleotide level; 98.5%~100% for cps2J gene, 98.7%~100% for gdh gene, and 99.5%~100% for ef gene at the amino acid level, respectively). It’s suggested that the cps2J, gdh and ef genes of the 3 human derived S. suis 2 strains isolated in Hunan Province have high homology with other S. suis 2 strains isolated in mainland China and abroad, and have a low degree of variation in the gene sequences.
收稿日期: 2012-10-11
出版日期: 2013-06-19
通讯作者:
欧新华
E-mail: xhouteam@163.com
引用本文:
欧新华 张如胜. 3株人源猪链球菌2 型湖南株cps2J、gdh、ef基因进化分析[J]. 中国人兽共患病学报, 2013, 29(4): 369-374.
Xin-Hua OU Ru－sheng ZHANG. Phylogenetic analysis of cps2J, gdh and ef gene of 3 human-derived Streptococcus suis serotype 2 strains isolated in Hunan Province. Chinese Journal of Zoonoses, 2013, 29(4): 369-374.
2013, Vol. 29 
