Abstract:Abstract: In order to efficiently detect foot-and-mouth virus(FMDV) strains of various serotypes, a one-step real-time quantitative RT-PCR (rRT-PCR) method based on a TaqMan-MGB probe was developed。A conserved regions in the 3D gene of FMDV was choosed as the target for primers and TaqMan probe. The results indicated that the MGB rRT-PCR could specifically detected A, O, Asia I serotype of FMDV and was negative for CSFV,HPRRSV,PRRSV,PRV,PPV,PCV2 and JEV. The sensitivity of this assay was 10 times higher than the multiplex RT-PCR and its minimum detectable concentration were 83.4copies/μL of recombinant plasmid and 7.1fg/μL of template RNA. The Variation coefficients were less than 2% based on 5 replications of 4 samples. These results showed that the MGB rRT-PCR assay was sensitive, specific, stable and could be used for FMD diagnosis in clinical samples, epidemiological investigation and livestock safety monitoring.