Abstract:ABSTRACT: The immunodiagnosis of human cystic echinococcosis (CE) is a valuable instrument for clinical diagnosis and epidemiological surveillance of high-risk populations. In the present study, the diagnosis potential of the recombinant gene products (rAgB8/1, rAgB8/2, and rAgB8/3) from Xinjiang (China) isolate of Echinococcus granulosus in comparison with native AgB (nAgB) and purified hydatid cyst fluid (HCF) were evaluated by anti-IgG ELISA test. Total RNA was prepared from E. granulosus protoscoleces collected from a sheep hydatid cyst from Xinjiang Uygur Autonomous. Three AgB subunits were obtained by reverse transcription-polymerase chain reaction (RT-PCR). PCR products were sequenced and cloned into pGEX-3X vector. Expressed recombinant proteins were induced by isopropyl-β-D- thiogalactopyranoside (IPTG). HCF was prepared from fertile sheep-liver cysts fluid through the dialysis and precipitation. HCF was boiled and centrifuged to isolate nAgB. Prepared 5 antigens were applied for detecting antibodies of the sera from patients with different paraditosis and control subjects using ELISA. The native antigens presented higher sensitivity ( HCF, 90.8% and nAgB, 87.4%) than recombinant antigens ( rAgB8/1, 67.8%, rAgB8/2 78.2% and rAgB8/3,,59.8%)., additionally, rAgB8/2 is more sensitivity than rAgB8/1 and rAgB8/3 (P<0.05). The specificity presented by these antigens with 96.0%, 96.0%, 96.0%, 98.0% and 97.0%, respectively, did not showed significant differences statistically (P>0.05). Diagnostic efficiency of the above antigens was 93.5%, 91.9%, 82.8%, 88.7% and 79.6%, respectively. The results suggested that the diagnosis potential of the native antigens (HCF and nAgB) were superior to the recombinant antigens (rAgB8/1, rAgB8/2, rAgB8/3) for detecting cystic echinococcosis in anti-IgG ELISA test.