Abstract:Objective: The aim of the present study was to investigate the distribution of toxin genes and antimicrobial profiles of S. aureus strains isolated from retail chicken in Shaanxi Province. Methods: A total of 122 S. aureus isolates from retail chicken were tested for the prevalence of nine enterotoxin and four other exotoxin genes by polymerase chain reaction, and antimicrobial susceptibility with 14 antibiotics by the agar dilution method. Results: Of the122 strains of S. aureus from retail chicken, 59.84% were positive for one or more toxin genes, 25.41% isolates harbored pvl gene, 51.64% isolates harbored one or more ses genes, and sej (37.70%) was the most common pattern, 4.92% were positive for mecA gene . None of the isolates harbored see,seg,sei,ets and tsst-1 genes. A total of 20 toxin gene profiles were obtained, and sej (21.31%) was the most common profile, later were pvl(8.20%), sej+pvl(4.92%), seh+sej+pvl(3.28) and seh+pvl(3.28%). Of these S. aureus isolates,100.0% were resistant to at least one antimicrobial, 88.52% to three or more antimicrobials, resistance was most frequently observed to erythromycin (87.70%), followed by trimethoprim/sulfamethoxazole (81.97%), tetracycline(67.21%), amikacin(59.02%), ciprofloxacin (53.28%), oxacillin (52.46%) and amoxicillin/clavulanic acid (40.16%),while significantly fewer isolates were resistant to ampicillin (32.79%), chloramphenicol (27.05%), gentamicin(20.49%), cefoxitin(13.11%) and cefoperazone(2.46%). None of the isolates were resistant to vancomycin. Conclusion: Many S.aureus isolates from retail chicken in Shaanxi Province harbored multiple toxin genes and exhibited multiple antimicrobial resistances. The presence of S. aureus strains and MRSA in retail chicken poses a potential threat to consumers’ health. Relevant department should strengthen hygiene management of the chicken products.
收稿日期: 2012-07-09
出版日期: 2012-11-20
基金资助:后稷学者讲座教授奖励计划项目;中央高校基本科研业务费专项资金资助;中国博士后科学基金项目
通讯作者:
王新
E-mail: xinwang7516@yahoo.com.cn
引用本文:
王新. 陕西省市售鸡肉中金黄色葡萄球菌的毒力基因检测及药敏分析[J]. 中国人兽共患病学报, 2012, 28(11): 1076-1080.
xin wang. Toxin Genes Detection and Antimicrobial Susceptibility Test of Staphylococcus aureus Isolated from Retail Chicken in Shaanxi Province. Chinese Journal of Zoonoses, 2012, 28(11): 1076-1080.