Abstract:Abstract: We conducted expression of cecropin A from the Musca domestica and identified its activity in vitro. Housefly cecropin A mature peptide gene was cloned and pET32a-mc plasmid was constructed.The vector pET32a-mc was transformed into BL21 (DE3). After induction with IPTG, the Trx&MC fusion protein was purified. The Trx&MC fusion protein was digested with pepsin in buffer(Ph1.3). The hydrolyzate was analyzed with tricine-SDS-PAGE. The antibacterial activity of MC was detected by liquid growth inhibition. Results indicated that MC in vitro has strong and long duration antibacterial activity. Thioredoxin, as a molecular partner, could affect MC biological activity and reduce toxicity to bacteria, suggesting that MC molecules will have a good prospect of application.
收稿日期: 2012-06-08
出版日期: 2012-11-20
基金资助:国家自然科学基金
通讯作者:
王炜
E-mail: hnwangwei@msn.com
引用本文:
王炜. 家蝇Cecropin A分子体外表达及抗菌活性鉴定[J]. 中国人兽共患病学报, 2012, 28(11): 1098-1101.
wei wang. Expression and identification of cecropin A from the Musca domestica. Chinese Journal of Zoonoses, 2012, 28(11): 1098-1101.