Cloning, expression and characterization of a gene encoding translocase of the outer mitochondrial membrane 34 (TOM34) in Schistosoma japonicum
HONG Yang1, LI Xue-zhen1, 2, FU Zhi-qiang1, SHI Yao-jun1, LU Ke1, YANG Jian-mei1, ZHU Chuan-gang1, YUAN Chun-xiu1, LI Hao1, LI Xiang-rui2, LIN Jiao-jiao1
1.Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences / Key Laboratory for Animal Parasitology, Ministry of Agriculture, Shanghai 200241, China; 2.College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China
Abstract:A full-length cDNA encoding the Schistosoma japonicum translocase of outer mitochondrial membrane 34 (SjTOM34) was first isolated from 42-day-schistosome cDNAs. The cDNA had an open reading frame (ORF) of 1 083 bp and encoded 360 amino acids. Real-time quantitative RT-PCR analysis revealed that the expression of SjTOM34 was a little higher in 35-day schistosomes than worms in other stage. The SjTOM34 was subcloned into pET28(+) and expressed as both inclusion bodies and supernatant in Escherichia coli BL21 (DE3) cells. Western blotting showed that the recombinant SjTOM34 (rSjTOM34) had good immunogenicity. Immunolocalization analysis found that SjTOM34 was distributed all over the worms of S. japonicum. It’s suggested that SjTOM34 may be important in schistosome, and further investigations are required to fully understand the function of this molecule.
洪炀,李学珍,傅志强,石耀军,陆珂,杨健美,朱传刚,苑纯秀,李浩,李祥瑞,林矫矫. 日本血吸虫TOM34基因的克隆和表达分析[J]. 中国人兽共患病学报, 2013, 29(2): 122-128.
HONG Yang, LI Xue-zhen, FU Zhi-qiang, SHI Yao-jun, LU Ke, YANG Jian-mei, ZHU Chuan-gang, YUAN Chun-xiu, LI Hao, LI Xiang-rui, LIN Jiao-jiao. Cloning, expression and characterization of a gene encoding translocase of the outer mitochondrial membrane 34 (TOM34) in Schistosoma japonicum. Chinese Journal of Zoonoses, 2013, 29(2): 122-128.
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