Detection of aminoglycoside resistance gene in Escherichia coli isolates from ducks and its dissemination mechanism
CHEN Yan-jie1, PEI Ya-ling2, PAN Yu-shan1, WU Hua1, LIU Jian-hua1, YUAN Li1, DU Xiang-dang1, MENG Chun-ping1, HU Gong-zheng1
1. College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, China; 2. Zhengzhou College of Animal Husbandry and Engineering, Zhengzhou 450011, China
Abstract:The aim was to elucidate the molecular mechanisms of resistance of E. coli isolates from duck against aminoglycosides and the dissemination mechanism of this resistance. Broth microdilution method was applied to investigate the drug-resistant phenotypes of E. coli isolates (n=27) from ducks, and the aminoglycoside resistance genes including aminoglycoside-modifying enzyme genes and 16S rRNA methylase genes in these isolates were detected by PCR and sequencing. Through plasmid conjugation, characteristics of resistance gene transfer were observed. The method of gene cloning was used to investigate the genetic environment of the rmtB gene. The results indicated that 23, 19, 19, and 18 of 27 isolates were resistant to apramycin, gentamicin, amikacin and neomycin, with resistance rates of 85.2%, 70.4%, 70.4%, and 66.7%, respectively. The rmtB gene were detected in 13 of 27 E. coli isolates and both rmrB and aac(3)-IV genes were found in 6 strains. All five transconjugants were obtained and seriously resistant to apramycin, gentamicin, amikacin and neomycin (MIC≥128 μg/mL) and positive for rmtB gene. Resistances to aminoglycoside antibiotics and cross-resistance in E. coli isolates from ducks were very serious. The rmtB gene was responsible for the resistance to aminoglycoside antibiotics in the tested strains and the horizontal transfer of this resistance may mainly be mediated by plasmid conjugation.
陈燕杰,裴亚玲,吴华,潘玉善,刘建华,苑丽,杜向党,孟春萍,胡功政. 鸭源大肠杆菌氨基糖苷类耐药基因的检测与传播扩散机制[J]. 中国人兽共患病学报, 2013, 29(2): 138-141.
CHEN Yan-jie, PEI Ya-ling, PAN Yu-shan, WU Hua, LIU Jian-hua, YUAN Li, DU Xiang-dang, MENG Chun-ping, HU Gong-zheng. Detection of aminoglycoside resistance gene in Escherichia coli isolates from ducks and its dissemination mechanism. Chinese Journal of Zoonoses, 2013, 29(2): 138-141.
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