Immune effect of T lymphocytes from Plasmodium vivax-infected patients stimulated by recombinant PvMSP1-19 antigen in vitro
LI Guang-you1, 2, XIA Hui1, TAO Zhi-yong1, CHEN Yong1, FANG Qiang1
1.Department of Pathogen Biology, Bengbu Medical College, Bengbu 233030, China; 2.Department of Laboratory, the Second People Hospital, Wuhu 240000, Chna
Abstract:In order to study the effect of T cells from Plasmodium vivax (P.vivax)-infected patients which were stimulated with PvMSP1-19 in vitro, the peripheral blood mononuclear cells (PBMCs) isolated from P. vivax-infected patients was stimulated with PvMSP1-19 protein, and cultured in rIL-2 containing media to generate PvMSP1-19 activated T cells. After 7 days of culture, PvMSP1-19 was used to restimulate the activated T cells for 24 hours. Then the cells were collected for detecting intracellular cytokines by Flow cytometry (FCM), and the cells-proliferations of T cells labeled by CSFE was also analysed by FCM. The percentages for IL-4 + T cells among activated CD8+ T cells that were stimulated with PvMSP1-19 was distinctly higher than that in the unrestimulated control group (P<0.05). Meanwhile, PI (proliferative index) of CD8+ T cells that were stimulated with PvMSP1-19 was distinctly higher than that in the unrestimulated control group (P<0.05). In conclusion, the recombinant PvMSP1-19 protein could effectively stimulate the proliferation of CD8+ T cells and the production of IL-4 secreted by CD8+ T cells.
李光友,夏惠,陶志勇,陈勇,方强. PvMSP1-19重组蛋白体外对间日疟患者外周血T细胞免疫功能的影响[J]. 中国人兽共患病学报, 2013, 29(2): 175-178.
LI Guang-you, XIA Hui, TAO Zhi-yong, CHEN Yong, FANG Qiang. Immune effect of T lymphocytes from Plasmodium vivax-infected patients stimulated by recombinant PvMSP1-19 antigen in vitro. Chinese Journal of Zoonoses, 2013, 29(2): 175-178.
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