Mice immune response to Echinococcus granulosus egG1Y162 vaccine
LIU Xiao-xia1,2,MA Hai-mei1,2,ZHU Ming1,ZHANG Li-na1,2, CAO Chun-bao2,ZHOU Xiao-tao1,DING Jian-bing1,2
1.School of Basic Medicine, Xinjiang Medical University, Urumqi 830011, China; 2. Base to Foster a State Key Laboratory for Major Disease Research in Xinjiang, the First Affiliated Hospital of Xinjiang Medical University, Urumqi 830011, China
Abstract：The purpose of this study was to investigate the immunological effects induced by the egG1Y162 hydride vaccine antigen in mice. The experimental group, GST group, FCA group and NS group of mice were injected with egG1Y162 vaccine, GST, FCA and NS. Sera were collected to determine the levels of IgG using ELISA. The proliferation of splenic T lymphocytes was determined by MTT method. Then the cells were collected and stained by PI and AnnexinV-FITC, and then analyzed by FCM to obtain the apoptotic rate of splenic T lymphocytes. It was found that the specific IgG response was induced during the second week after immune and continued to increase until 10th week. The highest titer of specific antibodies was 1;25 600 in sera of mice immunized with the egG1Y162 antigen. Level of IgG in the sera of mice increased obviously on 2-10 weeks, reaching the highest level on 10th week. As demonstrated by the MTT assay, the egG1Y162 vaccine could specifically stimulate the proliferation of splenic T lymphocytes in mice. In the statistical analysis, the levels of proliferation stimulation after vaccination in the vaccine group were significantly higher than those in the control, adjuvant, and normal groups. Apoptotic rate of splenic T lymphocytes in immunization groups with or without ConA stimulation was obviously lower than that of PBS group (P<0.01). Apoptotic rate of splenic T lymphocytes in each group with ConA stimulation was greatly higher than that in the group without ConA stimulation (P<0.01). The Echinococcus granulosus egG1Y162 vaccine could induce high titers of antibodies and stimulate spleen lymphocytes’ specific proliferation activation. Results indicated that the specific antibodies response and splenic proliferation activation collaboratively promote the production of humoral immune and cellular immune responses, which jointly induce the production of immune responses.
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2013, Vol. 29 
