1.College of Animal Science and Technology, Agricultural University of Hebei, Baoding 071000, China; 2. Beijing Key Laboratory for Prevention and Control of Infectious Diseases in Livestock and Poultry, Institute of Animal Science and Veterinary Medicine, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China
Abstract：To investigate prevalence and antimicrobial susceptibility of Campylobacter jejuni and Campylobacter coli in pig gut, samples from Beijing suburban pig farms and slaughter house were collected for Campylobacter isolation. After enrichment, the samples were simultaneously examined for the genus Campylobacter, the species of C. jejuni and C. coli by triplex PCR. Isolation and identification of Campylobacter strains were performed from the PCR-based positive samples. Finally, the Campylobacter isolates were tested for antibiotic resistance by antimicrobial susceptibility assay. In this study, a total of 241 samples of pig intestinal contents and anal swabs were screened by triplex PCR. Totally, 178 (73.9%) and 5 (2.1%) samples showed positive for C. coli and C. jejuni respectively. Of all the Campylobacter positive samples by PCR, 63 (35.4%) C. coli isolates were isolated and identified while no C. jejuni isolate was obtained. Antimicrobial susceptibility of 63 C. coli isolates was evaluated with 23 antibiotics. The results showed that the C. coli isolates were highly sensitive to chloramphenicol (100%), antimicrobial (93.65%), doxycycline (91.48%), gentamicin (82.54%), and amoxycillin (82.54%); while significantly resistant to cefazolin (98.41%), cefixine (92.06%), nalidxic acid (92.06%), streptomycin (90.48%), ciprofloxacin (90.32%), norfloxacin (88.89%), and penicillin (87.30%). Prevalence of multiple antibiotics resistance in the C. coli isolates was observed with a high percentage of 11-17 antibiotics resistance. This study indicated that C. coli was the most commonly detected Campylobacter species in pig gut with significant resistance to multiple antibiotics.
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2013, Vol. 29 
