Abstract:To screen the molecules binding dengue II virus expressed in Aedes albopictus midgut tissue and C6/36 cells, C6/36 cells were infected with dengue II virus, and the virus were collected and purified. The total proteins of Aedes albopictus midgut tissue and membrane proteins of C6/36 cells were extracted and analyzed using12% SDS-polyacrylamide gel (PAGE). After electrophoresis, the proteins were transferred to a nitrocellulose membrane, and virus overlay protein binding assay (VOPBA) was carried out using an anti-dengue virus monoclonal antibody; one specific band of 30 kDa occurred after VOPBA of the proteins from Aedes albopictus midgut tissue incubated with the virus, while the negative control group did not show this specific band. Two specific bands of 30 kDa and 40 kDa occurred after VOPBA of the proteins from the cells incubated with the virus, while the negative control group did not show these specific bands; the 30 kDa molecular binding with dengue type 2 was successfully expressed in both Aedes albopictus midgut tissue and C6/36 cell, and its identification of amino acid sequence and biological functions is in progress.
潘京,朱娉婷,郑学礼. 白纹伊蚊中肠组织和C6/36细胞与登革病毒相互作用蛋白的筛选[J]. 中国人兽共患病学报, 2013, 29(5): 447-451.
PAN Jing,ZHU Ping-ting,ZHENG Xue-li. Screening of proteins interacting with dengue virus from Aedes albopictus midgut tissue and C6/36 cells. Chinese Journal of Zoonoses, 2013, 29(5): 447-451.
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