新型肝穿刺建立原发性肝多房棘球蚴病小鼠模型
卞志远1 ，马少波1 ，夏海洋1 ，赵阶峰1 ，唐景霞2 ，张示杰1 ，彭心宇1 ，杨宏强1
1.石河子大学医学院第一附属医院肝胆外科,石河子832002;
Establishment on mouse model of primary hepatic Echinococcus multilocularis by transhepatic injection
BIAN Zhi-yuan1 ,MA Shao-bo1 ,XIA Hai-yang1 ,ZHAO Jie-feng1 ,TANG Jing-xia2 ,ZHANG Shi-jie1 ,PENG Xin-yu1 ,YANG Hong-qiang1
1.Hepatobiliary Surgery, The First Affiliated Hospital, Medical College, Shihezi University, Shihezi 832002, China;
摘要 目的 探讨新型肝穿刺方法建立原发性肝多房棘球蚴(Echinococcus multilocularis, E.m)小鼠模型。方法 将120只12w龄健康雌性小鼠随机分为切开皮肤经腹壁肌层肝穿刺接种、开腹肝穿刺接种和经皮肝穿刺接种3组,每组40只。20%乌拉坦腹腔注射麻醉后,分别对3组小鼠进行肝脏注射E.m组织混悬液,制备模拟原发性肝多房棘球蚴小鼠模型。结果 3组小鼠的存活率依次为85%、75%、80%(P>0.05),肝脏E.m感染率依次为70.6%、70.0%、62.5%(P>0.05),胸腔误穿率依次为0%、0%、25%(P<0.01)。结论 切开皮肤经腹壁肌层肝穿刺接种可成功制备模拟原发性肝多房棘球蚴小鼠模型,既简化了开腹接种的操作,又减少了经皮肝穿刺误穿胸腔的发生率。
关键词 ：
多房棘球蚴 ,
动物模型 ,
肝脏 ,
肝穿刺
Abstract ：The purpose of this research was to investigate a new method of transhepatic injection to establish primary Echinococcus multilocularis (E.m) model in liver of mouse. The 120 healthy female mice of 12-week-old were randomly divided into three groups as follows: transhepatic injection through skin incision and musculi abdominis, laparotomic transhepatic injection, and percutaneous transhepatic injection (40 in each group). By giving intraperitoneal injection with 20% Urethane, every mouse was injected with E.m tissue suspension respectively through three different operational methods to establish mimic primary E.m model in liver of mouse. Results showed that the survival rates of mice of the three groups were 85%, 75% and 80%, respectively (P>0.05); the E.m infection rates in liver of mouse were 70.6%, 70.0% and 62.5%, respectively (P>0.05); and the incidence of inadvertent puncture of pleural were 0%, 0% and 25%, respectively (P<0.01). A primary E.m model in liver of mouse can be successfully established by the method of transhepatic injection through skin incision and musculi abdominis, which was not only simplified the operation of laparotomic transhepatic injection but also reduced the incidence of inadvertent puncture of pleural following percutaneous transhepatic injection by transhepatic injection through skin incision and musculi abdominis.
Key words ：
Echinococcus multilocularis
animal model
liver
transhepatic injection
收稿日期: 2012-12-02
基金资助: 国家自然科学基金项目(No. 81160199);国家科技部支撑计划项目(No. 2009BAI82B06)联合资助
通讯作者:
杨宏强,Emailhongq01@sina.com
引用本文:
卞志远，马少波，夏海洋，赵阶峰，唐景霞，张示杰，彭心宇，杨宏强. 新型肝穿刺建立原发性肝多房棘球蚴病小鼠模型[J]. 中国人兽共患病学报, 2013, 29(5): 452-455.
BIAN Zhi-yuan,MA Shao-bo,XIA Hai-yang,ZHAO Jie-feng,TANG Jing-xia,ZHANG Shi-jie,PENG Xin-yu,YANG Hong-qiang. Establishment on mouse model of primary hepatic Echinococcus multilocularis by transhepatic injection. Chinese Journal of Zoonoses, 2013, 29(5): 452-455.
链接本文:
http://www.rsghb.cn/CN/10.3969/cjz.j.issn.1002-2694.2013.05.006 或 http://www.rsghb.cn/CN/Y2013/V29/I5/452
[1]Proietti S, Abdelmoumene A, Genevay M, et al. Echinococcal cyst[J]. Radio Graphics, 2004,24(3): 861-865. DOI: 10.1148/rg.243035173
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