Abstract：The molecular biological analysis method was used to clarify molecular characteristics of the echovirus type 6 (ECHO6) virus isolated in Longyan City in Fujian and to recognize its relationship with the isolates in the rest of world. The virus were isolated from patients cerebrospinal fluid (CSF) specimens with rhabdomyosarcoma (RD) cells, and the serotypes were classified through neutralizing test on positive isolates during the outbreak of children viral encephalitis in Changting County in Fujian Province,May of 2010. The complete VP1 sequences of the 4 isolates in Longyan were successfully amplified and compared with other ECHO6 isolates available from GenBank, and then they were constructed a VP1 gene phylogenetic tree. In 4 sequenced strains, the length of VP1 coding region was 866 bp (1 strain) and the others were 867 bp (3 strains). The homologies of these 4 strains were higher than 99%. The VP1 nucleotide sequence homology matrix analysis showed that 4 strains of the virus had the highest homology with Shandong virus strain E6/SD/sewage/081226/2-2. Phylogenetic analysis showed that these four ECHO6 strains were in the same branch of C2 genotype. In conclusion, ECHO6 was the pathogen of this children viral encephalitis outbreak in Changting, Fujian, and the genotype is C2 subtype.
陈前进，曹春远，吴水新，杨秀惠，罗招福，何云，廖亦红. 一起儿童病毒性脑炎暴发肠道病毒ECHO6型分离株VP1基因特征分析[J]. 中国人兽共患病学报, 2013, 29(5): 523-526.
CHEN Qian-jin,CAO Chun-yuan,WU Shui-xin,YANG Xiu-hui,LUO Zhao-fu,HE Yun,LIAO Yi-hong. Analysis on the gene characteristics of ECHO 6 strains isolated from an outbreak of children viral encephalitis. Chinese Journal of Zoonoses, 2013, 29(5): 523-526.
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2013, Vol. 29 
